Comparison of survival percentage of three varieties of pistachio subordinateneathneath aridity and salinity urgency showed there is a expressive contrariant natant pistachio varieties tolerance to aridity and salinity requisite. In aridity urgency, Sarakhs and Akbari showed the primary and last tolerance respectively (Figure1 A), time the results of salinity urgency On the opposite and prove that Akbari and Sarakhs showed the last and primary tolerance, respectively (Figure1 B).
Effect of aridity and salinity in Proline full
Free proline full was analyzed in leaves of urgencyed and administer pistachio varieties seedlings. Underneath aridity and salinity urgency requisites, proline full increased expressively in comparison succeeding a time administer plants. Underneath urgency requisites, diversity of Sarakhs exhibited inferior fulls of proline than that of the two another varieties. Proline full increased, expressively in Akbary veriety laagered to aridity and salinity urgency from 3 and 9 days succeeding opening of urgency. Surprisingly, proline full of the Badami and Sarakhs varieties increased queer 9th day succeeding urgency. When compared succeeding a time administers meanwhile, twain aridity and salinity urgency increased proline full in Badami and Akbari varieties not-absolute to administers (Figure- 2).
The effects of salinity and aridity urgencyes on completion phenolic full shown in Fig. 3. Of the three varieties, the primary phenolic full was narrative in Sarakhs and Badami varieties subordinateneathneath aridity urgency in 9th day succeeding rouse urgency. However, in salinity queer Sarakhs and Akbari varieties expressively surpassing at 9 days succeeding tenor (Figure 3).
Changes in H2O2 full and lipid peroxidation of pistachio seedlings
To summon the contrariantial oxidative loss brought environing salt and aridity urgencyes in three pistachio varieties, we measured the fulls of H2O2 and lipid peroxidation in pistachio leaves (Figure. 4&5).
Under salinity urgency requisites, H2O2 fulls increased from 3rd day succeeding urgency and in 9th day Badami and Sarakhs verities entertain primary total of H2O2. However, negative Sarakhs, the raze of H2O2 was expressively increased from 3rd day succeeding urgency in Badami and Akbari varieties subordinateneathneath aridity urgency requisites. In importation, the raze of H2O2 in salt urgency was surpassing than to aridity urgency (Figure. 4).
Similarly, lipid peroxidation assessed by MDA full in pistachio varieties leaves expressively increased 3 days succeeding salt and 6 days succeeding aridity urgency in all varieties negative Badami (Figure 5). The raze of lipid peroxidation was surpassing in 9th day than to other days in all urgency requisites.
Changes in activities of antioxidant enzymes
We measured the enzymatic air of SOD, PPO and CAT in leaves of seedlings of three varieties endanger succeeding a time salinity and aridity urgency. As shown in Table 1. salinity and aridity urgency increased the air of SOD, PPO and CAT in leaves of three varieties. More precisely, the SOD air of Badami in salinity urgency and Akbari in aridity urgency were surpassing than that of administer. All varieties exhibited a homogeneous increasing in SOD air in aridity requisites succeeding rouse urgency. However, in salinity urgency Sarakhs diversity has primary SOD air in 3rd day succeeding urgency and in Badami and Akbari varieties the primary SOD air observed in 6th day. In importation, the SOD air raze was correspondent to aridity and salinity urgency, negative Badami in salinity urgency (Table. 1).
Similarly, the air of PPO in leaves of three varieties increased upon tenor succeeding a time Aridity and salinity. Furthermore, Akbari so showed surpassing PPO air in aridity and salinity urgency, when compared succeeding a time other varieties. PPO air in all of varieties in aridity urgency increased from 3rd day to 9th and in salinity urgency primary air for this enzyme narrative in 3rd day succeeding urgency and then decreased it. The results showed that PPO air razes in aridity urgency were surpassing than salinity urgency (Table. 1).
The air of CAT showed that varieties of Badami and Akbari entertain primary CAT air subordinateneathneath aridity urgency in 6th and 3rd respectively. Varieties of Sarakhs and Badami showed primary CAT air in 6th day and Akbari prove in 3rd day and then decreased. In salinity urgency all of varieties showed primary CAT air in 6th day. Also, CAT air raze to aridity and salinity urgency (Table. 1).
Under Aridity and Salinity urgency requisites, varieties of Badami in aridity and Akbari in aridity and salinity urgency exhibited surpassing fulls of discerptible sugar at 9th day. Increasing in discerptible sugar in aridity and salinity urgency rouseed from 6th day and total that expressively increased in Badami and Akbary than to Sarakhs diversity. Negative Badami, in other varieties were discerptible sugars full in salinity urgency surpassing than aridity urgency (Fig. 6).
Glycine-betaine full was expressively increased in salinity and aridity than administer. The primary full of Glycine-betaine resulted in Badami in dought urgency to 6th day and Akbari in salinity and aridity urgency at 9th day. In salinity rouse of Glycine-betaine increasing was from 3rd day for Sarakhs and Badami and 9th day for Akbari. But, in aridity urgency rouse of Glycine-betaine increasing was from 3rd day for Badami and 6th day for Akbari and Sarakhs. According to the results, total of Glycine-betaine in Sarakhs is expressively inferior than Akbari and Badami (Fig. 7).
Anthocyanin pigments were extracted from the Pistachio leaves seedlings and roughly measured spectrophotometrically. Anthocyanin full was expressively increased in salinity and aridity urgency than administer requisite. The primary full of Anthocyanin in aridity urgency showed in 9th day in all tested varieties, time in salinity urgency there was this increasing in 3rd day for Sarakhs and in 6th day for Akbari and Badami. Compare of three varieties at administer requisite showed that Akbari and Sarakhs entertain primary and last anthocyanin full (Fig. 8).
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